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When it comes to legislating against dog attacks, the focus should be on dog owners, not dogs.
The interactions of nineteen peptide substrates and fifteen analogous peptidomimetic glycolate inhibitors with human peptidylglycine α-amidating monooxygenase (PAM) have been investigated. The substrates and inhibitors are the prohormones of calcitonin and oxytocin and their analogues. PAM both secreted into the medium by and extracted from DMS53 small lung carcinoma cells has been studied. The results show that recognition of the prooxytocin and procalcitonin peptide sequences by the enzyme extends more than four and five amino acid residues, respectively, from their C-termini. This substrate sequence recognition is mirrored by increased inhibitor potency with increased peptide length in the glycolate peptidomimetics. Substitution of the C-terminal penultimate glycine and proline residues of prooxytocin and procalcitonin and their analogues with phenylalanine increases the enzyme binding affinity. However, this changes the binding mode from one that depends on peptide sequence recognition to another primarily determined by the phenylalanine moiety, for both the substrates and analogous glycolate inhibitors.
From parents who refuse to vaccinate their kids for fear of any side effects to the endemic abuse of pharmaceuticals, many Australians make bad medical decisions in spite of established advice. How can healthcare providers and brands help them make more informed choices about their wellbeing? FULL ARTICLE
With 27% claiming they ‘cannot survive’ without it and 75% having at least one cup daily, it’s fair to say that Australians love their coffee, leading many to seek the highest quality and most consistent brew to suit their complex flavour profiles. How have they become such discerning drinkers?